Plant Tissue Culture - an overview | ScienceDirect
Plant Tissue Culture. Plant tissue culture is defined as culturing plant seeds, organs, explants, tissues, cells, or protoplasts on a chemically defined synthetic nutrient media
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Plant Tissue Culture. Plant tissue culture is defined as culturing plant seeds, organs, explants, tissues, cells, or protoplasts on a chemically defined synthetic nutrient media
Endemic flora, particularly trees, can come to represent the entirety of a culture, and their health an allegory of a society’s strength. Earlier this year Patrut et al. (
In vitro culture-based micropropagation of plants has gained a lot of importance over the last three decades as this technique is considered as a viable tool for large-scale production of commercially important plants of medicinal, horticulture, plantation crops, and pharmaceutical importance (Thorpe 2007).Similarly, several plant species
1. Introduction. The initiation of in vitro studies of plant cells and tissue culture dates back to 1902, when Gottlieb Haberland presented a “totipotency” hypothesis that each cell has all the genetic information needed to produce a perfect plant [1,2].Differentiated cells in plants are able to re-enter the cell cycle, proliferate and regenerate tissues and
Linum usitatissimum L. is a plant used by human since ancient times. Presently flax has both industrial and nutritional significance. The programmes of restoration of flax cultivation and processing are implemented to renew the importance of this plant for agriculture and economy. Genetic engineering methods and techniques of plant tissue
We could isolate and identify two predominant bacterial isolates from date palm in vitro cultures, i.e. Bacillus subtilis and B. cereus [10], B. cereus and B. subterraneus [40]. On the other hand
Microbial contaminants can be introduced both with the explants used to initiate plant tissue cultures and at every stage of the tissue culture process in the laboratory [3, 4, 33]. However, it is often difficult to determine the exact source or sources of contamination from a visible assessment of the contaminated
Two genes active in the early steps of flavonoid biosynthesis were isolated from a citrus cDNA library, and their expression patterns were analyzed during citrus (Citrus unshiu Marc.) fruit development in relation to flavonoid contents.The respective cDNA clones from chalcone isomerase (CHI, CitCHI) and flavanone 3-hydroxylase (F3H, CitF3H) are
Human life depends on plant biodiversity and the ways in which plants are used are culturally determined. Whilst anthropologists have used phylogenetic comparative methods (PCMs) to gain an
Plants’ secondary metabolism is an important source of medicinal and industrial products. Even though natural ecosystems are still the most important font of this kind of substance, excessive harvesting of spontaneous flora can act as a direct cause of biodiversity loss. Different technologies are used for in vitro production which, in addition
Plant tissue culture can be broadly defined as the in vitro aseptic maintenance of cells, tissues or organs under defined conditions. The pioneering developments for sustaining isolated plant cells date back to the early 1900s [].Haberlandt already introduced the concept of totipotency , which refers to the unique genetic
Analysis of BY-2 plant cell culture RNA-seq data (Yang et al., 2015; Yang et al., 2017) revealed high expression levels of enzymes in both the TCA cycle and gluconeogenesis, with average transcripts per million (TPM) for enzymes in the TCA cycle being 368 and glycolysis 717. However enzymes specific to the glyoxylate cycle, malate
The importance of maize (Zea mays L.) to global agriculture, world economy, and food security is widely known and increasing. Current maize breeding programs are deeply integrated with recent and rapid technological advances in genome sequencing, computational biology, and new genotyping and phenotyping technologies.
Hemacytometer. Image analysis. Microscopy. Microspore culture. For in vitro culture of plant and animal cells, one of the critical steps is to adjust the initial cell density. A typical example of this is isolated microspore culture, where specific cell densities have been determined for different
Plant tissue culture can be broadly defined as the in vitro aseptic maintenance of cells, tissues or organs under defined conditions. The pioneering developments for sustaining isolated plant cells date back to the early 1900s [].Haberlandt already introduced the concept of totipotency , which refers to the unique genetic
Compton ME (1994) Statistical methods suitable for the analysis of plant tissue culture data. Plant Cell Tissue Organ Cult 37:217–242 Compton ME (2010) Elements of in vitro research. In: Trigiano RN, Gray DJ (eds) Plant cell culture, development and biotechnology. CRC, Boca Raton, FL, pp
Introduction. Haploids are the fundamental ploidy-level type, from which diploids or tetraploids are easily produced via chromosome doubling. Microspores as gametic cells that have undergone androgenesis are able to produce haploid plants through in vitro culturing. This microspore culture provides a useful way for plant breeders to
Bacterial microorganisms which are latent in in vitro cultures can limit the efficiency of in vitro methods for the conservation of genetic resources. In this study we screened 2,373 accessions from the in vitro sweetpotato germplasm collection of the International Potato Center in Lima, Peru for bacteria associated with plantlets in tissue
In this context, several requirements have led plant scientists to use real-time RT-PCR methods rather than northern blotting. First, the analysis of more than ten genes by northern blotting is a fairly tedious and repetitive work, as several identical blots have to be prepared (Dong et al.,
The importance of maize (Zea mays L.) to global agriculture, world economy, and food security is widely known and increasing. Current maize breeding programs are deeply integrated with recent and rapid technological advances in genome sequencing, computational biology, and new genotyping and phenotyping technologies.
The main aim of in vitro micropropagation is to obtain true-to-type plants to maintain the characters of mother plant, but, during in vitro culture, there is a chance of genetic changes which are commonly known as “somaclonal variations” (Larkin and Scowcroft 1981). The occurrence of genetic defects as a result of somaclonal variation in
Hemacytometer. Image analysis. Microscopy. Microspore culture. For in vitro culture of plant and animal cells, one of the critical steps is to adjust the initial cell density. A typical example of this is isolated microspore culture, where specific cell densities have been determined for different
During the late 1980s and early 1990s, plants and plant suspension cell cultures were proposed as alternative production systems (Hiatt et al., 1989; Fischer et al., 1999). In particular, the scalability of plant-based systems combined with the low cost of plant cultivation was predicted as a major driver to reduce manufacturing
Stress and defense responses in relations to the production of plant secondary metabolites. Stress response in plants comprises repertoire of molecular, cellular cross-talk and signaling responses initiated through the detection of specific or combined biotic or abiotic stress effect that may result in the induction of SM [].Plants immune
Abstract. Somaclonal variation (SC) in plants regenerated from tissue culture, via organogenesis or somatic embryogenesis, is frequently associated with abnormalities in the content of deoxyribonucleic acid (DNA), viz., aneuploidy and polyploidy. Flow cytometry (FCM) using the nucleic acid-specific fluorochrome propidium iodide has proven to be
Analysis of BY-2 plant cell culture RNA-seq data (Yang et al., 2015; Yang et al., 2017) revealed high expression levels of enzymes in both the TCA cycle and gluconeogenesis, with average transcripts per million (TPM) for enzymes in the TCA cycle being 368 and glycolysis 717. However enzymes specific to the glyoxylate cycle, malate
Main conclusion Plant tissue culture as an important tool for the continuous production of active compounds including secondary metabolites and engineered molecules. Novel methods (gene editing, abiotic stress) can improve the technique. Humans have a long history of reliance on plants for a supply of food, shelter and, most importantly,
Cultivating native bacteria from roots of plants grown in a given environment is essential for dissecting the functions of the root microbiota for plant growth and health with strain-specific